WebAug 16, 2024 · Measuring the binding affinity to ACE2 using the Biacore S200 (GE Healthcare) resulted in an affinity of 17 nM for RBD-WT and 16 pM for RBD-62 binding to ACE2, a 1,000-fold increase in affinity ... WebOct 12, 2015 · We report here that a commonly studied DUB mutation, in which the catalytic cysteine is replaced with alanine, can dramatically increase the affinity of some DUBs for ubiquitin. Overexpression of these tight-binding mutants thus has the potential to sequester cellular pools of monoubiquitin and ubiquitin chains.
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WebDec 31, 2011 · Previously, a 3-million-fold affinity maturation process was achieved in a protein-protein interaction composed of a variant T-cell receptor fragment and a bacterial superantigen. Here, we present the molecular basis of this affinity increase. Using X-ray crystallography, shotgun reversion/replacement scanning mutagenesis, and … WebJan 18, 2024 · Affinity Publisher is a great, affordable alternative to Adobe InDesign. And much like InDesign, Affinity Publisher also supports pre-made templates. With Affinity … delivery architects london
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